In ovo feeding improves energy status of late-term chicken embryos

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In ovo feeding improves energy status of late-term chicken embryos.

Maintenance of glucose homeostasis during late-term embryonic development is dependent upon the amount of glucose held in reserve primarily in the form of glycogen in the liver and upon the degree of glucose generated by gluconeogenesis from protein first mobilized from amnion albumen and then from muscle. Insufficient glycogen and albumen will force the embryo to mobilize more muscle protein t...

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In ovo electroporation is an excellent method to ectopically induce or inhibit gene expression in chicken embryos and to study the in vivo function of genes during embryonic development. However, the application of electroporation in ovo to date is limited to an early stage of incubation (< stage 20). In older embryos (> stage 22), the vitelline and allantoic vessels have developed extensively ...

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Gene transfer into older chicken embryos by ex ovo electroporation.

The chicken embryo provides an excellent model system for studying gene function and regulation during embryonic development. In ovo electroporation is a powerful method to over-express exogenous genes or down-regulate endogenous genes in vivo in chicken embryos(1). Different structures such as DNA plasmids encoding genes(2-4), small interfering RNA (siRNA) plasmids(5), small synthetic RNA olig...

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ژورنال

عنوان ژورنال: Poultry Science

سال: 2005

ISSN: 0032-5791

DOI: 10.1093/ps/84.5.764